CyberinfraV1, Main, Exploration, bibRecord, 000346

Functional Identification of the General Acid and Base in the Dehydration Step of Indole-3-glycerol Phosphate Synthase Catalysis*

Identifieur interne : 000346 ( Main/Exploration ); précédent : 000345; suivant : 000347

Functional Identification of the General Acid and Base in the Dehydration Step of Indole-3-glycerol Phosphate Synthase Catalysis*

Auteurs : Margot J. Zaccardi [États-Unis] ; Eric M. Yezdimer [États-Unis] ; David D. Boehr [États-Unis]

Source :

The Journal of Biological Chemistry [ 0021-9258 ] ; 2013.

RBID : PMC:3772182

Abstract

Background: Bacteria require the enzyme indole-3-glycerol phosphate synthase for the production of Trp.

Results: Glu-51 and Lys-53 are identified as the base and acid acting in the dehydration step of enzyme catalysis.

Conclusion: Ring closure and dehydration steps are catalyzed by distinct active-site surfaces.

Significance: Enzyme inhibitors targeted against these active-site surfaces may serve as novel antibiotics.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3772182

DOI: 10.1074/jbc.M113.487447
PubMed: 23900843
PubMed Central: 3772182

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en"><p><bold>Background:</bold>
 Bacteria require the enzyme indole-3-glycerol phosphate synthase for the production of Trp.</p>
<p><bold>Results:</bold>
 Glu-51 and Lys-53 are identified as the base and acid acting in the dehydration step of enzyme catalysis.</p>
<p><bold>Conclusion:</bold>
 Ring closure and dehydration steps are catalyzed by distinct active-site surfaces.</p>
<p><bold>Significance:</bold>
 Enzyme inhibitors targeted against these active-site surfaces may serve as novel antibiotics.</p>
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<name sortKey="Yezdimer, Eric M" sort="Yezdimer, Eric M" uniqKey="Yezdimer E" first="Eric M." last="Yezdimer">Eric M. Yezdimer</name>
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Functional Identification of the General Acid and Base in the Dehydration Step of Indole-3-glycerol Phosphate Synthase Catalysis*

Functional Identification of the General Acid and Base in the Dehydration Step of Indole-3-glycerol Phosphate Synthase Catalysis*

Source :

Abstract

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